James Schneider Ph.D.
Assistant Professor, Chemical Engineering; Faculty of Biomedical Engineering; Biotechnology and Environmental Processes; Biosensing and BioMEMS; Cellular and Molecular SystematicsOur work involves tailoring interfaces for biological applications, including biomaterials, bioseparations, and drug delivery. A major effort is in the design and synthesis of lipid-like molecules with peptide headgroups ("peptide amphiphiles") for use in liposomal drug and gene delivery. The peptides we are currently studying have a substantial fraction of peptide nucleic acid (PNA), a synthetic polyamide with pendant DNA nucleobases. In collaboration with Prof. Bruce Armitage, we are utilizing the specific recognition of complementary PNA strands to effect liposome transport from hydrogels at a specific, tunable temperature or pH. PNA-conjugated amphiphiles are also being explored as shuttles for DNA across cellular delivery barriers in gene therapy. Finally, we are also developing PNA-conjugated amphiphiles as a means to deliver PNA itself as an anti-sense inhibitor of gene expression.
We are also using the atomic force microscope (AFM) to characterize polymeric films and hydrogels used in biosensors and drug delivery. Using bioactive AFM probes in tapping mode, we can depth-profile polymeric films under various solution conditions and locate embedded antibodies or ligands along with their local binding force. In collaboration with Aristech, we are also mapping the mechanical properties of blended polymers using force modulation techniques.
EducationB.S. 1992, University of Wisconsin; Ph.D. 1998, University of Minnesota
Important ArticlesRecent Publications
J. SCHNEIDER, Y.F. Dufrêne, W.R. Barger, and G.U. Lee. "Mechanisms of Imaging Contrast in Phase-Separated Langmuir-Blodgett Bilayers", submitted to Biophys. J., 2000
H. Bianco-Peled, Y. Dori, J. SCHNEIDER, M. Tirrell, L.P. Sung, and S. Satija. "Structural Study of Langmuir Monolayers Containing Lipidated Poly(ethylene glycol) and Peptides", submitted to Langmuir, 2000.
J. SCHNEIDER and M. Tirrell. "Direct Measurement of Molecular-Level Forces and Adhesion in Biological Systems", in Bioadhesive Drug Delivery Systems: Fundamentals, Novel Approaches, and Development (Mathiowitz, Chickering, Lehr, eds.) pps. 223-260, 1999.
J. SCHNEIDER, P. Berndt, K. Haverstick, S. Kumar, S. Chiruvolu, and M. Tirrell. "Force and Adhesion Measurements between Hydrogen-Bonded Layers of Glycine-Functionalized Amphiphiles", J. Am. Chem. Soc. 120: 3508-3509, 1998.
By this Researcher