Fractal Analysis of Binding Kinetics on Biosensor Surfaces

Authors

Ajit Sadana Department of Chemical Engineering, University of Mississippi

Publication Date

4/13/04

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Abstract

Biosensors find increasing applications in different areas such as the detection of biological agents, chemicals, explosives, food safety, environmental, and energy applications. The main reasons for the growing popularity of the biosensor-based applications are the simplicity of use, monitoring of the reactions in real time and radiolabel free detection of the binding or breakdown of complexes. There is a continual need to enhance the sensitivity, selectivity, stability, regenerability, and decrease the response time of the biosensor. Also, it is significant to obtain kinetic and dissociation rate coefficients for analyte–receptor interactions in order to obtain better physical insights into functionally dissimilar processes they are involved in. An analysis of the interaction wherein the receptor is immobilized on the sensor chip of a surface plasmon resonance (SPR) biosensor and the corresponding analyte is in solution is an initial step in this direction. This is an in vitro application. However, one recognizes that the receptor may not be immobilized in real-life application in the human body (for e.g., cellular interactions).